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Pseudomonas syringae uses the syringe-like Type III Secretion System (T3SS) to inject Type III Secreted Effector (T3SE) proteins into the plant host. The primary role of T3SE proteins is believed to be the suppression of innate immunity. Recognition of effector proteins by the host Resistance (R) proteins results in the initiation of defense responses, including the hypersensitive response (HR). The pathogen can then evolve or acquire new T3SE proteins that evade the host defense response.

Given these dual roles of virulence and defense induction, T3SEs provide excellent tools to probe innate immunity in plants. My lab focuses on identifying new components in innate immunity and understanding their roles in defense, using a wide variety of experimental approaches.

Karl J. Schreiber, Ilea Chau-Ly and Jennifer D. Lewis. 2021. “What the wild things do: Mechanisms of plant host manipulation by bacterial type III-secreted effector proteins.” Microorganisms 9: 1029 DOI: 10.3390/microorganisms9051029

Karl J. Schreiber and Jennifer D. Lewis. 2021. “Identification of a putative DNA-binding protein in Arabidopsis that acts as a susceptibility hub and interacts with multiple Pseudomonas syringae effectors.” Molecular Plant-Microbe Interactions 34(4): 410-425. DOI: 10.1094/MPMI-10-20-0291-R

Karl J. Schreiber, Jana A. Hassan and Jennifer D. Lewis. 2021. “Arabidopsis Abscisic Acid Repressor 1 (ABR1) is a susceptibility hub that interacts with multiple Pseudomonas syringae effectors.” Plant Journal 105(5): 1274-1291. DOI: 10.1111/tpj.15110.

Maёl Baudin, Eliza C. Martin, Chodon Sass, Jana A. Hassan, Claire Bendix, Rolin Sauceda, Nathan Diplock, Chelsea D. Specht, Andrei J. Petrescu and Jennifer D. Lewis. 2021. “A natural diversity screen in Arabidopsis thaliana reveals determinants for HopZ1a recognition in the ZAR1-ZED1 immune complex.” Plant, Cell & Environment 44(2): 629-644. DOI: 10.1111/pce.13927.

Bálint Csörgő, Lina M. León, Ilea J. Chau-Ly, Alejandro Vasquez-Rifo, Joel D. Berry, Caroline Mahendra, Emily D. Crawford, Jennifer D. Lewis, Joseph Bondy-Denomy. 2020. “A minimal CRISPR-Cas3 system for genome engineering.” Nature Methods 17: 1183-1190. DOI: 10.1038/s41592-020-00980-w.

Jana A. Hassan, Ilea J. Chau-Ly and Jennifer D. Lewis. (2020) “High-throughput identification of resistance to Pseudomonas syringae pv. tomato in tomato using seedling flood assay.” Journal of Visualized Experiments 157: e60805.

Yuan Chen, Claire Bendix and Jennifer D. Lewis. (2020) “Comparative genomics screen identifies microbe-associated molecular patterns from Candidatus Liberibacter sp. that elicit immune responses in plants”. Molecular Plant-Microbe Interactions 33: 539-552.

Maёl Baudin, Karl J. Schreiber*, Eliza C. Martin*, Andrei J. Petrescu and Jennifer D. Lewis. (2020) "Structure-function analysis of the immune receptor ZAR1 reveals key molecular interactions for activity." Plant Journal 101: 352-370. *co-second authors

Karl J. Schreiber and Jennifer D. Lewis. (2019) “Protein acetylation in pathogen virulence and host defense: In vitro detection of protein acetylation by radiolabeled acetyl Coenzyme A.” Methods in Molecular Biology Plant Innate Immunity 1991: 23-32.

Jana A. Hassan, Roberto de la Torre-Roche, Jason C. White, and Jennifer D. Lewis. (2018) “Soil mixture composition alters Arabidopsis susceptibility to Pseudomonas syringae infection.” Plant Direct 2(2): e00044. DOI: 10.1002/pld3.44.

Claire Bendix and Jennifer D. Lewis. (2018) “The enemy within: phloem-limited pathogens.” Molecular Plant Pathology 19(1): 238-254. DOI: 10.1111/mpp.12526

Yunchen Gong, Darrell Desveaux, David S. Guttman, and Jennifer D. Lewis. (2017) A practical guide to Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq). Methods in Molecular Biology Biological Networks and Pathway Analysis 1613: 1-20.

Maёl Baudin, Jana A. Hassan, Karl J. Schreiber, and Jennifer D. Lewis. (2017) “Analysis of the ZAR1 immune complex reveals determinants for immunity and molecular interactions.” Plant Physiology 174(4): 2038-2053.

Jana A. Hassan, Yanming Jane Zhou, and Jennifer D. Lewis. (2017) “A rapid seedling resistance assay identifies wild tomato lines that are resistant to Pseudomonas syringae pv. tomato race 1.” Molecular Plant-Microbe Interactions 30(9): 701-709.

Karl J. Schreiber*, Maёl Baudin*, Jana A. Hassan, and Jennifer D. Lewis. (2016) Die Another Day: molecular mechanisms of effector-triggered immunity elicited by type III secreted effector proteins. Seminars in Cell and Developmental Biology 56: 124-133. *co-first authors DOI: 10.1016/j.semcdb.2016.05.001.

Jennifer D. Lewis, Mike Wilton, G. Adam Mott, Wenwan Lu, Jana A. Hassan, David S. Guttman and Darrell Desveaux. (2014) Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. PLoS One 9(12): e116152. pdf of article

Jennifer D. Lewis, Timothy Lo, Patrick Bastedo, David S. Guttman and Darrell Desveaux. (2014) The rise of the undead: pseudokinases as mediators of effector-triggered immunity. Plant Signaling & Behavior 8(12): e27563. pdf of article

Jennifer D. Lewis, Amy Huei-Yi Lee, Jana A. Hassan, Janet Wan, Brenden Hurley, Jacquelyn R. Jhingree, Pauline W. Wang, Timothy Lo, Ji-Young Youn, David S. Guttman and Darrell Desveaux. (2013) “The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a.” Proceedings of the National Academy of Sciences USA 110(46): 18722-18727. pdf of article

Amy Huei-Yi Lee, Brenden Hurley, Corinna Felsensteiner, Carmen Yea, Wenzislava Ckurshumova, Verena Bartetzko, Pauline W. Wang, Van Quach, Jennifer D. Lewis, Yulu C. Liu, Frederik Börnke, Stephane Angers, Andrew Wilde, David S. Guttman and Darrell Desveaux. (2012) “A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion.” PLoS Pathogens 8(2): e1002523. pdf of article

Jennifer D. Lewis, Janet Wan, Rachel Ford, Yunchen Gong, Pauline Fung, Pauline Wang, David S. Guttman and Darrell Desveaux. (2012) “Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2.”  BMC Genomics 13: 8. pdf of article

Jennifer D. Lewis, Amy Huei-Yi Lee, Wenbo Ma, Huanbin Zhou, David S. Guttman and Darrell Desveaux. (2011) “The YopJ superfamily of type III effectors in plant-associated bacteria.”  Molecular Plant Pathology 12(9): 928-937. pdf of article

Jennifer D. Lewis, Ronald Wu, David S. Guttman and Darrell Desveaux. (2010) “Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein.” PloS Genetics 6(4): e1000894. pdf of article

Jennifer D. Lewis, David S. Guttman and Darrell Desveaux. (2009) “The targeting of plant cellular systems by injected type III effectors.” Seminars in Cell and Developmental Biology 20(9): 1055-1063. pdf of article

Jennifer D. Lewis, Karl Schreiber and Darrell Desveaux. (2009) “The needle and the damage done: type III effectors and the plant immune response.” In Molecular Plant-Microbe Interactions, edited by K. Bouarab, N. Brisson and F. Daayf. Oxon: CAB International, p. 179-210.

Jennifer D. Lewis, Wasan Abada, Wenbo B. Ma, David S. Guttman and Darrell Desveaux. (2008) “The HopZ family of Pseudomonas syringae type III effectors require myristoylation for virulence and avirulence functions in Arabidopsis thaliana.” Journal of Bacteriology 190(8): 2880-2891. pdf of article